|
KMID : 0603820100160030193
|
|
Journal of Experimental & Biomedical Science
2010 Volume.16 No. 3 p.193 ~ p.196
|
|
|
Forensic STR Analysis of Mixed Chimerism after Allogeneic Bone Marrow Transplantation
|
|
Eom Yong-Bin
|
|
|
Abstract
|
|
|
|
Multiplex PCR-based short tandem repeat (STR) analysis is considered as a good tool for monitoring bone marrow engraftment after sex-mismatched allogeneic transplantation and provides a sensitive and accurate assessment of the contribution of both donor and/or recipient cells in post-transplantation specimens. Forensic STR analysis and quantitative real time PCR are used to determine the proportion of donor versus recipient each contained within the total DNA. The STR markers were co-amplified in a single reaction by using commercial PowerPlex® 16 system and AmpFlSTR® Identifiler® / Yfiler® PCR amplification kits. Separation of the PCR products and fluorescence detection were performed by ABI PRISM® 3100 Genetic Analyzer with capillary electrophoresis. The GeneMapper™ ID software were used for size calling and analysis of STR profiles. Extracted DNA was quantified by the Quantifiler™ Human DNA / Y Human Male DNA Quantification Kit. The intent of this study was to analyze the ratio of donor versus recipient cells in the post-transplant peripheral blood, spleen, lung and kidney specimens. Specimens were taken from the traffic accident male victim who had been engrafted from bone marrow female donor. Blood and spleen specimens displayed female donor DNA profile. Kidney specimen showed male recipient DNA profile. Interestingly, lung tissue showed mixed profiles. The findings of this study indicate that the forensic STR analysis using fluorescence labeling PCR combined with capillary electrophoresis is quick and reliable enough to assess the ratio of donor versus recipient cells and to monitor the mixed chimeric patterns.
|
|
|
KEYWORD
|
|
|
Short tandem repeat(STR) analysis, Allogeneic transplantation, Mixed chimerism
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|